细胞DNA定量分析技术相关文献

  • 1、HIGH-RESOLUTION IMAGE CYTOMETRY ON SMEARS OF NORMAL ORAL MUCOSA: A POSSIBLE APPROACH FOR THE EARLY DETECTION OF LARYNGOPHARYNGEAL CANCERS(HEAD & NECK 2004年1月)

    作者:Andreas Neher, Andreas Gschwendtner

    关键字:咽喉癌,咽喉粘膜脱落细胞

    摘要:

    The purpose of this study was to investigate the possibility of identifying laryngopharyngeal cancers by nuclear chromatin texture feature analysis of cell nuclei from mucosal scrapings obtained from clinically and cytologically noncancerous areas of the soft palate in patients with cancer. The collective consisted of 68 controls and 77 cases of laryngopharyngeal carcinomas. After Feulgen staining, 3000 cell nuclei were automatically measured using a high-resolution image analyser (CytoSavant Oncometrics, Vancouver, BC, Canada). Texture features were extracted for calculation of a discriminant function, which allows the two groups to be distinguished. Two parameters allowed the two populations to be distinguished. The classifier reached an overall performance of 72.7% sensitivity, 82.4% specificity, a positive predictive value of 80.5%, a negative predictive value of 75.1%, and an area under the receiver operating characteristics (ROC) curve of 0.7754. Our work shows that subtle changes in the chromatin distribution in cell nuclei from ostensibly normal cells in the vicinity of carcinomas are demonstrable in the oral cavity of patients suffering from laryngopharyngeal cancers. It may be possible to develop this method into a valuable clinical tool to reduce the high rate of delayed diagnosis of oral and laryngopharyngeal cancers.


  • 2、用细胞DNA 定量分析方法进行宫颈癌普查的临床研究(诊断病理学杂志 2004年10月)

    作者:孙小蓉, 汪键

    关键字:宫颈癌筛查

    摘要:

    目的探索用细胞DNA定量分析方法进行宫颈癌普查,以提高普查的工作效率和准确性.方法对参与宫颈癌普查的20000名妇女用宫颈刷取材,进行液基薄层制片,分别进行巴氏染色和DNA染色.对巴氏染色片做常规细胞学检查,对DNA染色片进行自动扫描诊断.结果对常规细胞检查结果在LSIL以上病例和全自动DNA倍体分析系统检查可见异倍体细胞或异倍体细胞峰的病例,建议进一步做阴道镜检查以及宫颈活检.1 016名妇女做了病理活检.以病理诊断结果为标准,计算出细胞DNA定量分析方法在筛查GIN3以上宫颈病变的敏感性为82%,特异性为71%,而常规细胞学方法则分别为52%和92%.结论细胞DNA定量分析方法在有条件的降低特异性的情况下,能明显提高宫颈癌普查的阳性检出率.


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